Smita Bhutda

5 Questions 9 Answers 0 Followers

Questions related from Smita Bhutda

Do anyone know better method to increase efficiency of transient transfection of k48R mutant ubiquitin plasmid?

I am trying to transfect brain endothelial cells using K48R mutant ubiquitin plasmid using Lipofectamin 2000 as well as lipofectamin 3000. But transfection efficiency is just 5%. Can some one...

01 January 2018 2,538 0 View

How do you use PKH dyes for intra-cellular endosome labeling?

Hi, I want to label adherent brain endothelial cells with pKH26 to visualize endosomes inside it. I tried staining it. However, my pKH26 staining is not consistent as well as it is not...

01 January 2018 3,628 1 View

Have anybody used far red fluorescence dye for staining bacteria for live microscopic imaging?

I am searching for a fluorescence far red dye to stain bacteria for live Microscopic Imaging. PSVUE is one of NIR (Near Infrared Dye) which stains anionic lipids but it is not compatible with PBS...

10 October 2016 1,911 0 View

Good Ubiquitin ligase inhibitor to block ubiquitination in HBMECs In Vitro

HI. I am trying to inhibit ubiquitination in Human Brain Endothelial Cells In Vitro using PYR-41 ubiquitin ligase inhibitor. But it is not inhibiting ubiquitination as lower concentration and at...

08 August 2016 8,341 2 View

IS there a staining for adherent and tight junction protein in fixed cells?

I have immortalized Human Brain Endothelial cells. I am trying to stain VE-Cadherin and Occludin in this PFA fixed cells. Instead of getting staining at cell periphery (On cell membrane), I am...

05 May 2015 8,375 9 View