What modifications do you make in ACSF for aged mice brain hippocampus recording?
Please if possible explain specifically for cutting and recording solutions?
I would recommend checking this website for aCSF recipes, especially for older animals https://www.brainslicemethods.com/ .
Transcardial perfusion of cold cutting aCSF before slice preparation and incubating slices in an interface chamber might enhance slice health.
good luck!
Dear Shahrukh Khanzada.
Deepak Subramanian recommendation is indeed a great direction, brainslicemethods.com offer serval ways to deal with this, deppending on the research question.
The simplest way that you could try is by using modified aCSF where NaCl is replaced by the same amount of Choline Chloride (I personally used approx. 125 mM) in the same way that you would use the non-modified aCSF as your cutting medium. Make sure that cutting solutions are ice-cold and of a sorbet consistency and you might be even able to skip the perfusion. If that works out in your hands, you might consider lowering the Choline concentration by dilluting it with non-modified aCSF (NaCl containing aCSF).
I would use a non-modified aCSF for recording.
Good luck!
- Badges
- Science topic