Deepak Subramanian

5 Questions 20 Answers 0 Followers

Questions related from Deepak Subramanian

How do you prevent spreading depression during intrahippocampal drug infusion in vivo?

I am trying to study the effect of a drug on dentate oscillations in urethane anesthetized rats, where I have a guide cannula + a tungsten wire lowered to the dentate gyrus. The tungsten wire...

15 February 2018 3,448 3 View

Is it possible to study changes in NKCC1 expression using acute hippocampal slices?

Changes in NKCC1 expression are seen in in vivo models of epilepsy and brain tissue from patients with drug-resistant seizures. Is it possible to study such changes using acute hippocampal slice...

23 October 2014 3,332 8 View

How do you prevent spreading depression in High K+ model?

I am currently using a high K+ model (8.5 mM K+ with 1mM CaCl2 and 1.3mM MgSO4) for inducing seizures in acute rat hippocampal slices (4-8wks old). Recently, we see a lot of spreading...

14 April 2014 7,283 8 View

Do high K+ induced seizures cause regulation of NKCC1 in adult brain?

Expression of NKCC1 and KCC2 appears crucial in triggering seizures in immature rats through breakdown of Cl- homeostasis, which apparently seem to stabilize with maturation. However, can...

01 March 2014 7,852 5 View

Why do we use dihydrate form of CaCl2 in ACSF preparation?

Most research articles and protocols mention using CaCl2. 2H20 in the preparation of ACSF. Is there a specific reason why we use CaCl2.2H20 in ACSF preparation rather than just using CaCl2? The...

17 May 2013 4,609 9 View