How do you normalise ddPCR mRNA expression using 3 housekeeper genes?

05 October 2022 1 7K Report

I have gene expression data from skin cells before and after treatment, and I want to normalise the expression of these two samples against the expression of 3 housekeeper genes.

How would I go about doing this?

Thanks in advance!

Stefanie Kretschmer

Hi Alex,

you can either choose the best (and most stable) single housekeeper gene for calculation or you can go with all of your genes.

In that case, I recommend reading the following site:

https://toptipbio.com/qpcr-multiple-reference-genes/

Dr. Steven Bradburn has a really great explanation with an example calculation.

Best,

Stefanie

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