How do you insert mCherry gene into chromosomal DNA of lactobacillus rhamnosus GG?

11 April 2019 0 5K Report

Hello I'm trying to insert mCherry gene into chromosomal DNA, just right before the stop codon of some housekeeping gene in Lactobacillus rhamnosus GG.

These are the candidate housekeeping genes, but I have no idea which one would work well.

ef-Tu: Elongation factor Tu

fusA2: Elongation factor G

gapA: Glyceraldehyde-3-phosphate dehydrogenase

groEI: Heat shock protein 60

groES: Chaperone

gyrB: DNA gyrase B

ileS: Isoleucine-tRNA ligase

ldhL: L-lactate dehydrogenase

pcrA: ATP-dependent DNA helicase

pfk: 6-Phosphofructokinase

pheS: phenylalanyl-tRNA synthase

recA: Recombinase

rpoA: α-subunit of RNA polymerase

rpoB: DNA-directed RNA polymerase

srtA: Sortase

can you please give me any advice?

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