I need to use these nitrocellulose membranes to bind Ab specific antigens, after immobilizing antibodies to it. Could you suggest any simple Ag - Ab pair to start with?
Helder André
you could try the flag M2 with the corresponding FLAG peptide. They should be available from sigma
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Christopher Bradley
Most commercial protein size markers contain recombinant proteins purified by the His-tag. You could use rainbow marker and anti-His antibody. Test this first by doing a western blot.
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