How do I circularize a plant mitochondrial genome using a de novo based approach?

11 December 2014 9,756 5 View

I want to merge two contigs together make one scaffold.Can anyone suggest some tools?

I have tried SSPACE were the contigs are extending but not merging in one to form a scaffold. For assembly i have used gsASSEMBLER. Kindly suggest.

08 September 2014 880 1 View

How to use SSPACE for scaffolding ?

I have an hybrid contigs of 454 + Illumina + SOLiD which is around 850 Mb. Now I want to create scaffolds using SSPACE with 20kb (3 libraries) of 454 paired end reads which have been converted...

07 August 2012 6,472 4 View

Hybrid assembly of complex genome

I have an hybrid data of 454 + Illumina + SOLiD which i have assembled into 157439 contigs covering around 1.3 GB genome. Now I want to create scaffolds using 20kb (3 libraries) 454 paired end...

06 July 2012 6,172 6 View

Hybrid Assembly of Complex Genomes

I am trying to assemble hybrid reads using PE and BAC reads. I have shredded the sequences 1Kb with 800bp overlap and I am assembling with Newbler. I am giving input of 1 GB but getting output of...

06 July 2012 6,114 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View