I am trying to think about a way to measure the protein translation rate for a single and specific protein that I am interested in. I've been thinking that Ribo-seq might be a good idea for this purpose given the fact that the mRNA sequence of the protein I am interested in its known, but it is an expensive experiment.
Of course, Western Blot will give me information about the increased or decreased production of my protein, but I am curious about knowing if any change in protein level takes place at the translational level or transcriptional level
Let me know what are your thoughts!
hi,
I hope, this article may help you.
Article Methods for monitoring and measurement of protein translatio...
Cheers!!!
Hi Simon,
a good standard for this would be pulse-chase experiments. Its quite easy and not expensive.
You might also want to try tetracycline for inhibition and then determine protein levels after wash-out at different time points.
Good luck
Boris
Boris Schmitz Thank you so much. Pulse Chase experiments like the one done with Azidohomoalanine?
I appreciate your reply!
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