What's the best fluorophore to use for KI67 analysis?

I would like to check proliferation of human CD8 cells (isolated from blood ) after few days of culture. I have a panel of antibodies and I want to add KI67 to analyse proliferation. Does anyone...

08 October 2020 4,763 3 View

Does anyone have a paper with the growth parameters (K, Loo and T0) of Bluefin tuna (Thunnus thynnus) in Mediterranean sea and Gulf of Mexico?

13 December 2015 9,549 7 View

Using proc Glimmix in SAS to fit a generalized logit model, how can I allow for correlations between the random intercepts for various outcome groups?

I am using proc glimmix in SAS to fit a multilevel model for a multinomial outcome with unordered response categories. Proc glimmix requires that you specify the group= option in the random...

12 March 2015 9,536 7 View

How many cells are needed for detection of 2-hydroxyethidium by HPLC-MS ?

I am preparing experiments to determine the superoxide content of cells (CHO and red blood cells) using hydroethidine and HPLC-MS (as described by Zielonka et al, Free radical in Biology and...

02 March 2015 2,066 4 View

Does anybody know the fluorescence duration of DCFDA and DHE ?

How long does the fluorescence of DCFDA and DHE last after fixation and mounting of the cells?

10 November 2013 9,232 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View