I dissolved indomethacin (Santa Cruz Biotech - SC-200503) in DMSO and prepared stock of 25 mg/mL
In my study, I inject 10 mg/kg i.p., injection volume 0,5 mL and I work with 250-300 g rats.
When I diluated the stock solution with saline (%0,9 NaCl), the diluated solution has been crystallized.
How can I avoid that?
Open for any suggestions.
My kind regards.
indomethacin 0.3578 mg/ml ( 0.001 M) dissolved in 0.01 M sodium carbonate in water . which was diluted 4-fold (0.00025 M) for injection (1.0 mg/kg(rat wt.), less than 0.3 ml/rat) is injected .
Hi, I agree with Madhukar. You can directly dissolve indomethacin in Na2CO3 (0.1mg/ml). Otherwise, you can avoid the use of DMSO on your stock solution using ethanol. Here are some infos of indomethacin solubility:
Santa Cruz: Soluble in ethanol (25 mg/ml), DMSO (25 mg/ml);
Cayman Chemical: Soluble in ethanol (6.73 mg/ml), DMSO (17.8 mg/ml), and dimethyl formamide (20.2 mg/ml);
Sigma Aldrich: Soluble in ethanol (50 mg/ml).
Cheers!
Madhukar Baburao Deshmukh and Clei Endrigo Piazza thank you for the advice
Kind regards,
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