How do you determine the position (-OH group) where a phosphorylation occurred in a sugar?

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Phillip Leigh Van der Peet

NMR is your best bet, the splitting caused by phosphorous should make the site of phosphorylation clear.

http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_05%3A_Structure_Determination_II/Section_5.8%3A_NMR_of_phosphorylated_molecules

Febby J Polnaya

31P NMR is your best tools to analyzed the site of phosphorylation.