Eloi Camprubí-Casas

6 Questions 6 Answers 0 Followers

Questions related from Eloi Camprubí-Casas

What would be the chemical shift of methyl groups bound to Fe or Ni atoms?

I have (at least) one organic compound in my aqueous samples which has a marked peak at ~0.1 ppm (see attached spectrum). I need to find out what it is. It is not TMS, since I use another internal...

10 June 2021 7,848 6 View

Is it possible to do a 'spike' test in 1H-NMR to identify a molecule when you suspect it's a gas?

I suspect I have some dissolved methane (and maybe propane) in my aqueous solutions. Please see attached spectrum (for both spectra: number of scans is 256; same receiver gain; same method; water...

07 May 2021 1,945 6 View

Is a fully (negatively) charged phosphate group a better or a worse leaving group compared to a protonated (neutral) phosphate group?

I have a reaction between an amine and acetyl phosphate. The results can be that the amine can be phosphorylated or acetylated. NMR experiments suggest that at high pH what happens mostly is...

25 July 2016 4,942 3 View

How to remove calcium ions from an aqueous sample?

I have aqueous samples heavily loaded (to saturation) with CaCO3 and CaOH2. I analyse my analytes (sugars) by derivatising them as alditol acetates followed by GCMS analysis. In theory, the last...

11 July 2016 6,664 3 View

Which external/internal standards would you use to aid in the quantification of small molecules using LC-MS?

This is long, sorry. I plan to use LC-MS to determine the concentration of several small molecules. In set of experiments these will be (i) derivatised sugar phosphates (using AEC) and in the...

07 April 2016 3,090 5 View

How do you determine the position (-OH group) where a phosphorylation occurred in a sugar?

Using a non-enzymatic reaction to phosphorylate small sugars (5C) so I assume there will be unspecificity and would like to know how to assign the phosphorylation site/s.

19 March 2015 7,559 2 View