Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

Why build a Leaning Tower of Lire?

What are the lessons to be learned from building a leaning tower of Lire?

03 January 2021 7,364 4 View

What are some of the applications of transparent wood?

Are there any particular uses for transparent wood other than visual appeal? The transparent wood is really cool to see, but what are the practical uses? Please provide examples. Happy New Year!

31 December 2020 2,479 4 View

Can light waves induce electricity through induction?

Can light waves induce electricity? I am unclear on this matter. Thank you.

30 December 2020 7,974 3 View

What are the benefits of applying Blockchain within any industry?

Is blockchain only for cryptocurrency? Thanks in advance.

28 November 2020 5,086 11 View

Is my Chi-square test valid?

18 November 2020 3,033 2 View

What is the maximum possible efficiency in Protein Extraction?

I would like to know what the highest possible percentage of Protein content within a Green Pea that can be extracted by manufacturing grade equipment? I would appreciate any research paper that...

29 March 2020 8,515 3 View

What acid and base is used in Protein extraction from Green Pea?

Hello, I would like inquire on the process of extraction of protein contents from the green pea by means of decantation. What I know is that a decantation is a separation process in which...

22 March 2020 586 4 View

Why color intensity increase during storage?

We found that the color intensity of an anthocyanin source extract (butterfly pea flower) increase during the first three days of storage. After that, the relatively rapid degradation occurred....

10 November 2018 9,167 0 View

Any suggestion where to find a detailed crop map of India?

Can anyone suggest a link to a map or database of detailed crop map (wheat, eggplant, pea, etc) of Gujarat India? I have checked some sources online but they are very coarse. I would want...

07 August 2018 3,113 0 View

What are the harmful impact of using DAP fertilizer on pigeon pea growth?

In India Di ammonium phosphate (DAP) is the most commonly used fertilizer for pigeon pea. But can someone tell me the authentic reports which proves that its causes harmful impact on the growth of...

04 May 2018 5,759 0 View

How do calculate trypsin inhibition of my sample using the method of Kakade et al., (1974)?

I am using the method outlined by the Kakade et al., (1974) to look at trypsin inhibition in peas. I am having trouble working the calculations back to find out how much is in my sample, using the...

10 January 2018 6,802 1 View

How do calculate trypsin inhibition of my sample using the method of Kakade et al., (1974)?

I am using the method outlined by the Kakade et al., (1974) to look at trypsin inhibition in peas. I am having trouble working the calculations back to find out how much is in my sample, using the...

31 December 2017 6,846 1 View

What is crude protein, crude fat, protein & fiber in pea food legume ?

What is crude protein, crude fat, protein & fiber in pea food legume ? Guys I haven't seen the role of crude protein, crude fat, protein and fiber in physiology of the plants starting from...

31 December 2016 7,887 1 View

Does anyone has a knowledge about rhizhobium tablets?

1. Their use in chick pea 2. Any research paper regarding the use of rhizobium tablets in any crop other than chick pea

06 July 2016 3,081 1 View

Could anyone provide an experienced protocol of Hematoxylin-eosin (HE) staining for pea aphid?

06 July 2016 5,208 0 View