How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

What is the best way to isolate multiple Salmonella serovars from a single sample?

I am trying to isolate multiple Salmonella serovars from a single food sample. I started by incubating the sample in a non-selective BPW enrichment and from there used multiple selective...

19 January 2021 8,365 4 View

No title

Hi there! I'm very new to r and trying to run a multi-level meta analysis using pre-calculated effect sizes. I've attached the datafile below. The script I used as a first step to fit the model...

12 January 2021 8,214 2 View

Non parametric alternatives to one-way ANOVA for 4 groups?

Hi, Currently assessing NDVI data (vegetation index, continuous data) against land type (4 groups, categorical variables). My data is not normal, or has equal variance (transforming hasn't...

14 December 2020 7,796 3 View

How to remove the jacketing from the midsection of an optical fibre?

I have a HCS fibre with a 200 micron diameter. I want to remove the jacketing in sections along its length so I can replace the cladding underneath and create an evanescent field sensor. Obviously...

13 December 2020 3,068 2 View

the lack of promotion bringing dissatisfaction on the employees?

Write a report with literature review and recommendations for The relationship between lack of promotion and dissatisfied employees to apply your management role to resolve for employees.

21 October 2020 6,698 2 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

Please help: R problems with forest plots ??

Hello, everyone! I am having trouble running my syntax for my forest plot. Below is the syntax I have used previously, with the changed data frame for my current analysis. For whatever reason, the...

27 June 2020 641 1 View

Does anyone know of a parent-report measure of child loneliness suitable for kids between 6 and 12?

This will be used in a study of children's well-being (we will only have access to parents).

23 March 2020 8,355 3 View

How to calculate substrate consumption rate?

Hello lovely people, It is my first question here, so I hope you can help me.. For an experiment of my thesis I provided pure cultures with different concentrations of lactate an monitored them...

26 February 2020 1,114 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

Can i use the " weight moisture" ( humidite ponderale in French) of plants to describe their efficiency in using water?

Weight moisture is : water content/ dry weight , if for example it is 4 it means that this plant have for each 1gam of dry mass 4 grams of water. can i use this to interpret the plant use of...

14 February 2021 5,784 1 View

Why (111) peak of ZeoliteX changes depending on dry or wet?

09 February 2021 6,307 3 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View