Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

Statistics: Controlling for pubertal status and gender?

Hi, I am not sure whats the best way to "control" for pubertal stage and gender in my analysis. Actually, puberty is classified in 5 stages (1=prepubertal to 5=fully grown). To make it easier and...

06 May 2020 9,363 3 View

How to make a 1500 watt high voltage(5-20KV) power supply?

I need to design and make a high voltage power supply to use in a lab made electrostatic precipitation with this specs: Input voltage: AC: 220 volt Output voltage: DC: 5-20 KV (Continuous...

09 April 2020 5,172 21 View

Experience with immunostaining with anti-Hepatocyte Specific Antigen (Hep Par-1) antibodies in frozen tissue?

Dear scientists, I would like to stain the formaldehyde-fixed, OCT-embedded human liver tissues with anti-Hep Par-1 antibodies (DAKO; clone OCH1E5) for IHC staining. Do you by any chance having...

20 February 2020 3,017 3 View

What causes wrinkles in thin films to disappear after heat treatment?

When I anneal the free standing thin film (>300 degree Celsius), wrinkles of the thin film disappear. What is the principle of this phenomenon? (This thin film is can be a single layer or a...

28 December 2019 8,410 4 View

What happens to the mechanical properties when annealing a metal thin film?

If the metal thin film is annealed, How do the mechanical properties such as tensile strength, hardness, Young's modulus change? The metal thin film is not alloy and thickness is less than 100...

21 August 2019 8,069 3 View

Induce Point Mutation in Microbes (RpoB gene for Rifampicin resistance)?

Hi, I try to make Rifampicin resistance microbes (e.g., Corynebacterium) using point mutation in RpoB gene. The doubling time of Corynebacterium is ~3.5hrs, and spontanoeus mutation rate for...

01 April 2018 6,253 2 View

Possible reasons causing ribosomal RNA size shift?

Hi all, I ran my RNA gel (formaldehyde gel containing MOPS buffer) to check the integrity of ribosomal RNA isolated from primary mouse hepatocytes. The 18s and 28s bands are good, which may...

26 November 2017 5,882 1 View

CDNA synthesis of norovirus

04 August 2017 2,580 3 View

I'm trying to fabricate some nanoparticles by n-butlLi, but I'm wondering how can I separate my Lithium helide salt from my nanopartcles in toluene?

see the reaction in the picture

10 July 2017 3,279 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View