How do you calculate IC 50 for inhibition of tyrosinase activity?

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Are there people who success to staining pax7 on mouse tissue (frozen, 4%PFA)?

I have never success a staining pax7, myoD, eMHY on mouse tissue. (DSHB antibody) When I did a heat-retrieval, staining is success but background was too strong. So I'm not sure if this is a good...

27 May 2024 1,597 1 View

Does anyone have papers regarding accounting for technology companies?

Preferable a paper that describes issues of accounting for R&D costs in technology firms and how managers use non-GAAP to provide a clearer picture of the core business.

11 March 2024 8,815 1 View

Any relevant research of the usefulness of non-GAAP figures in the field of Valuation?

Want to research the value relevance of non-GAAP figures in the field of company valuation. Especially interested in non-GAAP figures/measures like the adjusted EBITDA, and if there is any...

28 January 2024 7,516 1 View

How can I achieve uniform PR coatings on rectangular substrates?

I would like to achieve uniform PR(photoresistor) coatings as thin as 300 nm on a thick glass substrate. SU-8 PR is thinking of using with dilution. The size of the substrate is 160*120mm...

18 December 2023 4,457 2 View

The bolt destroyed my front mass threads ☹️ any advice?

The stainless steel bolt destroyed my front mass(AL7075) threads ☹️ any advice? this is for an ultrasonic transducer

09 November 2023 8,934 1 View

Can adenosine cross blood-brain barrier?

Hello. Recently, I want to inject the adenosine in vivo to see its effect on the behaviors. But I am not sure whether it can cross blood-brain barrier. If it could, I would choose easier...

27 October 2023 5,687 2 View

How to find the phase shift between the current transformer output and pwm signal generated my stm32?

I have a current transformer on the output of my inverter that is working as a feedback. From it i have a sinusoidal waveform that i want to find its phase shift in respect to pwm signal of...

04 June 2023 180 1 View

Is there a zero crossing detector inside the stm32 itself?

04 June 2023 4,386 0 View

How can I attach elemental chips on the target for sputtering?

Hello :) I would like to perform co-sputtering with elemental chips like the attached image. And I wonder how can I attach the chips on the target surface? * Is it okay if I just put it on? Or...

31 May 2023 7,350 16 View

Proof of the Hodge Conjecture?

Dear all, I think I have proven the Hodge conjecture, but it is difficult for me to say if what I did is error-free. I submitted it to various journals obtaining rejections based not on fatal...

28 May 2023 7,361 0 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View