I have transfected plasmids containing a portion of the promoter region of my gene upstream of the firefly luciferase gene and have used a NanoLuc expressing plasmid as my transfection control into mammalian cells. I have taken the measurements for the assay (2 plate readings) but I am unsure how to analyse the results. This is the 1st time I have used the NanoLuc system. Previous luciferase systems I have used take 3 luminescence readings, but the NanoLuc system only requires 2, so I don't know how to undertake the analysis. Any help would be great, thanks!