Hi Augustine! Generally speaking, most of the PCR reactions nowadays are performed using ready made kits - the reagents needed will be provided in stock solutions (i.e. buffers, MgCl2 solutions and dNTP solutions and the polymerases) - you have to mix them together and add your specific DNA primers and a DNA template to do the actual PCR reaction - all of these is also described in the following protocol: https://www.jove.com/science-education/5056/pcr-the-polymerase-chain-reaction
Good Luck!
Hi! Simply use ready-to-use PCR mixture, as Josef suggested. Most of molecular biology companies have quite broad range of such master mixes in their offer. The example of a mix from my country (not a commercial but an example): http://aabiot.home.pl/aabiop/PDF/PCR%20Mix%20100_EN.pdf
Thank you so much. Very helpful. Here, I am in as situation where the components are already sourced separately. I hope we shall be able to promote the purchase and use of the ready-to-use PCR mixtures, but in the meantime, I have to figure out on expected concentrations estimates.
So check the protocol/manual of your polymerase. The information about concentrations of each reagent for PCR is usually included. There is an example: https://tools.thermofisher.com/content/sfs/manuals/MAN0012027_TaqDNAPolymerase_recombinant_5_UuL_500U_UG.pdf
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