How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Seeds of wild Brassica rapa?

Hi, We are a team working on wild and cultivated plants and our stock miss of seeds of Brassica rapa from wild populations in Europe. We are based in Finland, we wonder if any laboratories in...

25 October 2020 857 3 View

Which proteins are continuously expressed (at stable levels) in mammalian monocytes and thus, suitable controls for Protein expression studies?

Hello! Currently I am testing whether an externally added agent affects the expression of only one particular protein or whether it generally inhibits protein synthesis due to its toxicity. For...

14 September 2020 6,359 3 View

Suggestions for an easy experiment on an inverted fluorescence microscope (students lab course)?

09 September 2020 290 3 View

Why it is that Disulfiram (DSF) only binds to zinc in cancer cells?

Good Afternoon, I understand that the zinc transporter proteins (ZIP7 and ZIP10) are both present in healthy and diseased cells. I learnt that zinc uptake is elevated in malignant cells...

09 October 2019 5,069 3 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Compare multiple means over time, which analysis?

I have a repeated measures design, looking at how sex hormones effect number of responses on a dichotic listening task, three times over the course of the menstrual cycle. Where it simply the...

14 April 2019 8,108 3 View

What is the best way to extract microRNA in postprandial blood?

Because of lipids in postprandial blood, I have some troubles to extract microRNA. How can I remove lipids without alter the quality of microRNA?

25 February 2019 2,781 3 View

What is the best way to know the quality of RNAm from plasma?

Hi everybody, I did extraction of total RNA on plasma samples (I am interested in circulating microRNA). How can I do to know if my sample are of good quality and if there are no...

01 October 2018 4,786 2 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

The hospitals reported rise in ICU admissions of young COVID patients - Is the new strain of COVID to be blamed?

05 January 2021 3,141 12 View

Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Why the western blot of HIF1alpha of the whole cell lysate shows intense band under normoxia without any induction of hypoxia?

I am working with oral cancer cell lines and trying to find the expressional changes of HIF1alpha under different experimental treatment. I am getting intense band of HIF1alpha in the western blot...

29 November 2020 5,707 3 View

RIPA content? any additional suggestion?

i am working on phosphorylation of NF-kB inducible in hypoxia using MDA-MB 231 cells. i have challenges getting my phosphoproteins through western blot. this is my RIPA content 50 mM Tris pH...

13 November 2020 3,356 3 View

Can anyone tell me what tools can be used for assessing psychological impact of postextubation patients in ICU?

Tools to assess this problem

12 November 2020 6,799 1 View

Can we detect neuroprotection, ischemic tolerance, ischemic pre conditioning using fmri ?

I want to know whether there are any non invasive techniques to detect neuroprotection in humans

02 October 2020 5,896 3 View

ELISA of isomer of glycolytic intermediate 2,3 DPG ?

Hello, I recently performed Kit based ELISA for 2,3 DPG on plasma samples of control and hypoxia treated rats. [CAT. NO. E1562Ra, Bioassay technology laboratory] However, the results I obtained...

07 September 2020 3,270 2 View

What are some in vitro experiments that can be performed to target hypoxia?

I want to show that my developed nanoparticle targets hypoxia but from doing research it seems like most hypoxic targeting related experiments are done in vivo. I'd like to be doing in-vitro...

16 August 2020 3,695 4 View

Can hypoxic air (1% O2, 5% CO2, 94% N2) buyed from the market?

I need hypoxic air (1% O2, 5% CO2, 94% N2) for hypoxia experiments, can I get/buy some hypoxic air from the market?

14 July 2020 5,471 2 View