How do I use stereotactic brain injection to administer multiple viruses at once in the same mouse?

More Ling Zhu's questions See All

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Why does the fatigue cracks initiate from surface to interior as the stress level decreases?

It is well known that low-cycle and high-cycle fatigue cracks initiate from the surface, while ultra-high cycle fatigue cracks initiate from the inside. I am very curious why this transformation...

17 January 2021 9,633 6 View

How to understand the chemo-mechanical coupling?

Recently, I came into contact with a new discipline named chemo-mechanical coupling. I found that chemo-mechanical coupling phenomena exist in many research areas, ranging from development of...

17 December 2020 1,065 3 View

Orthogonal array response vectors in poor scattering mmwave MIMO system?

Can anybody help in understanding how in a MIMO system with large (ideally infinite) number of transmit/receive antennas and poor scattering (e.g at mmWave), the transmit/receive array response...

17 December 2020 6,903 4 View

I am working on membrane distillation model in COMSOL and I have some problems coming in it?

I need to add a latent heat flux of evaporation to the hot side boundary, which is a function of the pressure difference between the cold side and the hot side boundary.However, the pressure at...

16 December 2020 9,890 1 View

How many ratio of simplicia powder : water I should add to make the infusion concentration 100%?

I am doing research to test antibacterial activity using plant leaves that I have mashed. How many ratio I should add between the simplicia and water to get 100% concentration in infusion technique?

06 December 2020 1,010 6 View

How much (sea)salt is needed to stop the turnover of the cold fresh water melting from the "ice"-poles?

The cold fresh water from the poles is going to block the warm water (Gulf) stream (heat transport). There is a theory that salt in water can conduct electricity. So it could start some electric...

19 September 2020 7,712 2 View

How to find the coordinate location of shared information in Google Drive?

Is it possible to know the coordinates of User Equipment which sent the data/information/file to another User Equipment on Google drive? Does drive has such features or option to identify the...

23 August 2020 3,315 3 View

Does Covid-19 effect certain parts of the brain?

Dear Colleagues, I like to know if Covid-19 effects the brain in general, depending on person's constitution or has Covid-19 a 'taste' for certain brain areas? Thank you for answering Kind...

27 July 2020 7,159 20 View

Human mesenchymal stem cell differentiation issue?

hello, i got a problem in the adipogenesis. The human MSCs were differentiated into adipocte. 10days later, the mutant MSCs and the WT MSCs were red oil stained and QPCR test. But there's a...

13 June 2020 5,169 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Marie Mortreux

Hi, it all depends on your volume and protocol. Even when injecting the olfactory bulb (bilaterally), i used 4 injection sites (same coordinates but different depths) without an issue. I also easily conducted studies where I performed injections in the OB and in the hypothalamus.

What are the sites you want to inject and what is your final volume?

Ling Zhu

Hi, I want to inject in the hypothalamus, but in the different brain region, such as LH; DMH; LPB; PVH. Marie Mortreux