i am researching on antioxidant activity of garcinia atroviridis and fenugreek seed at different temperatures and different concentration of methanol.
details on the research;
0.1 mM of DPPH solution is prepared
samples extract = 10g/100ml
procedure used;
1. 0.5 ml extract (supernatant) is added with 1 ml of 0.1 mM DPPH in methanol solution
2. Shake vigorously
3. Reaction time 30 minutes
4. Absorbance reading at 517 nm
i am curious do i have to make serial dilution of my samples to carry out this method?
1ml 0.004% solution of DPPH + 10 micro litter than 20 micro and so on to 100 micro liter should be prepared to evaluate IC50 value and to exactly predict DPPH Scavenging activity
Dear
Serial dilution only needed when you try to investigate the lc50 value, which is more acceptable way to introduce your antioxidant potential.
Good luck
Dear Umirah
The Idea of antioxidants activity depends on the estimation of Ic 50 , of course you need to prepare a series of diluted concentrations.
hi, may I know, if I am using crude extract (which 2g of plant sample dissolved in 20ml solvent), did my extract concentration be considered as 0.1g/ml? Then, If i were to make serial dilution for DPPH analysis, I just need to dilute it from the abovementioned crude extract concentration (0.1g/ml), right? I am still confused...
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