How do i tag any protein for ubiquitin mediated proteosomal degradation?

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Anyone explain the EDX Spectra and Elemental composition (at. % and Wt. %) of the dopant and composite. Why we need both the percentage?

Anyone explain the EDX Spectra and Elemental composition (at. % and Wt. %) of the dopant and composite. Why do we need both percentages? No one explains clearly in the research article....

27 February 2024 1,885 1 View

How to calculate the Atomic and Weight percentage from EDX? Any formula to calculate the at.% and Wt. %?

28 January 2024 3,085 3 View

How to calculate the Atomic and Weight percentage from EDX?

Kindly explain, how to calculate the elemental composition of weight and atomic percentage from EDX. Does anyone know the formula to calculate the at% and Wt%?

09 January 2024 1,575 3 View

Looking for a live technical webinar on VANET simulations with SUMO?

Join us for the NetSim Webinar on Jan 11, 2024, at 15:30 IST, focusing on vehicular ad hoc networks with NetSim & SUMO co-simulation. Learn about advanced techniques and applications in...

07 January 2024 1,602 1 View

XPS Standard book is available ??

Kindly send any one for Standard XPS book in the downloaded format.

13 December 2023 8,697 5 View

What is the conclusion obtained from weight and atomic percentages of elements present in the samples?

13 June 2023 1,709 0 View

What is the conclusion obtained from weight and atomic percentages of elements present in the samples?

13 June 2023 5,796 3 View

Due to different variation, XRD intensity peak goes to lower angle side and higher angle side means????. anyone explain clearly??

What is the physics mechanism behind that??

30 March 2023 6,643 2 View

How to add error value to experimental data (XRD, UV,PL,XPS,SEM)?

I need a clear explanation, How to add error values for all the experimental data?

01 February 2023 2,635 3 View

Does Subscription through journal publication is mandatory if we choose to opt golden open access?

Here's the case , For papers accepted for publication in (JES or JSS, )authors choose whether to publish their articles as open access.(ie via standard subscription mode) All articles accepted...

02 November 2022 1,593 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View