Dear colleagues!

I am starting experiments with monocytes isolated from PBMCs. My downstream application is flow cytometry.

I have tried accutase, trypsin, and ice-cold PBS + EDTA but the cells do not detach nicely. I don’t want to scrape them because of the downstream analysis. Should I maybe use non adherant (non treated) culture plates?

I would really appreciate your suggestions.

Thank you for your help!