12 June 2013 1 6K Report

I have data of LSV I want to convulate that data for data reliability.

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How to dissolve selenium nanoparticles powder?

After successfully synthesizing selenium nanoparticles from plants using water my next objective is to assess their antioxidant activity. However, I encountered an issue during the drying process...

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Why there is need to used catalyst during nanoparticles synthesis?

Good evening everyone I am working on green synthesis of selenium nanoparticles from leaf extract. The research article mentions that during selenium nanoparticle synthesis, ascorbic acid should...

24 September 2023 4,015 2 View

Why ascorbic acid used as standard for ABTS assay?

ABTS can dissolved in both water and organic solvent. for ABTS assay we can used trolox catechin and ascorbic acid as standard, but mostly ascorbic acid used as standard, and why. why ABTS assy...

09 July 2023 3,455 3 View

How to calculate plant extract concentration (that we want to use to perform experiment like 1mg/ml-5mg/ml) from mother plant extract?

i want to use 1mg/ml to 5mg/ml concnetration for antioxidant assay. my total drug concentration (mother drug ) is 12mg. how to calculate 1-5mg/ml con. from mother drug if total reaction volume is...

30 January 2023 9,656 2 View

How to calculate the standard deviation for total phenolic content?

i want to add my data on abstract for my research paper so how to calculate and plot the values in that and also from which value we have to calcualte the standard deviation

22 December 2022 7,017 1 View

How to calculate resolution from if retention factor and plate number only given?

coumn length is 50 cm and unretained marker takes 2 min to be detected. calculte the resolution for the peaks (A-B, B-C) how long must the column be in order to have baseline resolution? assume...

24 November 2022 8,732 1 View

How to calculate IC50 value for nitric oxide scavening assay?

i have done triplicate experiment for nitric oxide assay and i take 25-225ug/ml concnetration. and i got 3 absorbance value for each concnetration. but dont understand how to calculate IC 50 value...

01 September 2022 5,792 1 View

Why sometimes control not showing colour during experiment?

i am doing metal chelation assay for that i used iron (II) Chloride tetrahydrate and ferrozine. i take 0.1 ml iron (II) chloride tetrahydrate in test tube and added 0.2ml ferrozine as a control...

18 July 2022 3,301 0 View

I am doing hydrogen peroxide scavenging activity of plant extract . I am using 1/50 dilution of extract so do I need to take pure or diluted extract?

I am using 1/50 dilution of (50-250 microgram concentration) and 3 ml of H2O2 but absorbance of sample continuously increasing. Or some time negative absorbance also getting I don't understand...

15 April 2022 6,221 3 View

How to prepare 43mM hydrogen peroxide from 30% hydrogen peroxide chemical ?

Give me details calculation.

03 January 2022 8,346 1 View

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