I ordered custom Oligos from Invitrogen at a 25nmole synthesis scale dry, and now I would like to have a solution with a concentration of 10µM. What is the equation that I should use to solve this problem?
Take the number on nMol from the tube label or oligo information sheet and multiply by 10 to get the volume of TE needed to dissolve the oligo to a concentration of 100 µM. You can then further dilute this to 10uM. So if the synthesis is 49nm then add 490ul of TE to get a 100 µM solution. 25nM is the scale of synthesis and as each added base adds at less than 100% efficiency you cannot assume that you have 25nM of product
Seriously, just look at the oligo company website. Besides, it's easier to make a 100 microMolar stock and then dilute 1:10 to get a 10 microMolar working solution. You can't fit enough buffer in the original tube to dilute to 10 microMolar.
It is recommended to use TE buffer to dilute your custom oligo but we use water without trouble. We have a library of 450 primers/oligos. (Although we use IDT).
Your data sheet/vial should come with actual nmoles of oligo you got. Can be more or less than 25. Use (10*(nmoles of oliog))uL of water/TE to dissolve the oligo. This will give you a 100 uM stock. Which you can dilute 1:10 to get a 10 uM working stock.
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