I've tried to use a centrifuge to separate lipid-encapsulated nanoparticles(~15nm) from excess lipids by aspirating supernatant after the centrifugation at 3000 x g for 30min.
But since both of lipid-encapsulated nanoparticles and lipids are well-soluble in chloroform, it's not easy to remove free lipid molecules properly.
Is there anybody who can give me some advice about the centrifugation or other methods to remove free lipids???
I want to get lipid-encapsulated nanoparticles only............
Try using non- polar solvents like Dichloromethe or petroleum ether .I am assuming your nano particles would be polar and may not dissolve in Dichloromethan or /petroleum ether or vice versa.You may centrifuge to separate lipid portion as usual.
Thank you. Dr. Abdur Mirajkar.
But I think using non-polar solvents as you said would not be useful to separate lipids from the nanoparticles.
Because the nanoparticles are covered(encapsulated) with lipid molecules so that they tend to be like a lipid molecule and dissolved in iso-octane(polar index: 0.1) as well.
Any comments on that?
Regards...
Hi Won,
Do you see any density difference between nano particles and lipid particle?if yes, you can simply spin the sample without any solvent to see if nano particles are settling down.
I used dalysis membranes
http://www.spectrumlabs.com/dialysis/rc.html
to remove excess surfactants in nanoencapsulations, although in my case it was an aqueous solution. You could check if there are dalysis membranes for organic solvents.
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Virology
- Virus