Hi I am working on chicken Embryos and want to perform and ISH with HH16/21 and 25 Embryos. Unfortunately sometimes it happens that air bubbles trap in the Head, heart and vessels, which I would like to remove before doing the actual ISH procedure. I'm struggling to find a good way since I am intending to use a lot of embryos and especially it is difficult to remove it from the heart and vessels with forceps, without damaging the embryo.
In past ISH the bubbles didn't disappear when doing the O/N probe incubation at 65°C. I am not quite sure if it affects the actual binding.
Took me some time, but I solved my problem. I used a mouth pipette and a microinjection needle and applied negative pressure.
By doing so, I didn't damage the embryo and got rid of the air bubbles.
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