Johannes Wittig

3 Questions 4 Answers 0 Followers

Questions related from Johannes Wittig

Is it possible to stain Hematoxylin and Eosin prior to sectioning of tissue/organoid?

Slide chambers have limited space for a staining procedure and are often covered up by on or two sectioned samples. Because of this issue I was wondering if I could stain first and than section...

26 February 2018 3,884 4 View

How do I remove air bubbles from chicken Embryos HH 21/25?

Hi I am working on chicken Embryos and want to perform and ISH with HH16/21 and 25 Embryos. Unfortunately sometimes it happens that air bubbles trap in the Head, heart and vessels, which I would...

06 May 2016 1,463 1 View

Does anyone know a good way to quantify double positive cells in complete retina section?

My task is to quantify double positive cells in the retina. Currently I use and antibody against BrdU (Alexa 555) and a transgenic reporter gfap:nls-gfp. Unfortunately the transgenic reporter is...

29 June 2015 2,828 6 View