I am looking to quantitatively characterize the picoplankton (cell size range 0.2 - 2 µm) from lake water samples. A few questions here have dealt with the dual-filtration approach, using 2 and 0.2 µm filters and analyzing the retentate using fluorescence, which makes intuitive sense. Unfortunately, analysis of the 2-µm retentate under a microscope reveals some unknown amount of picoplankton is stuck in there, likely due to complex colony shape and the fact that any amount of clogging can probably hold them back. I welcome any suggestions on how to truly separate the picoplankton for analysis. Flow cytometry with imagery may be possible but may also be inaccessible, and apparently debris and bubbles can confound those results.
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