How do I rectify the standard curve in Cytokine Bead Array (Multiplex/Bioplex) ?

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What etchant should be used for Annealed 17-4 PH stainless steel sheet ??

I wish to determine microstructure of 17-4 PH stainelss steel sheet (annealed condition). What etchant should I use so that a clear microstructure can be found out ??

03 January 2021 9,836 2 View

What is the difference between PEEQ, PE and LE options in abaqus ??

I wish to find out thickness variation in my model, so for that which one I should choose among PEEQ, PE and LE ??

26 December 2020 6,542 2 View

Do you think selfishness and opportunism are key to the success?

Do you think selfishness and opportunism are key to the success? Dou you agree that principles, morals, ethics, intelligence etc. are only useless, show-words, even in the fields of science,...

24 December 2020 10,015 12 View

How to plot thickness contour plot and load diaplacement diagram in stretching simulation in abaqus ?

How can we plot thickness contour plot in stretching simulations in abaqus. Thickness contour plot means variation of thickness with time as stretching takes place. How can we plot load Vs...

10 November 2020 1,019 3 View

What are the methods to automatically annotate a large dataset of images?

Computer vision, Deep learning

22 October 2020 6,027 5 View

How can we measure the content of happiness in a person by applying various mathematical and soft computing techniques?

Here I just want to know about the actual parameters to measure the content of happiness in a person. With the help of these parameters a neural network can be generated and maintained to achieve...

22 September 2020 7,666 2 View

Pathway to large scale produce customised Solar PV complete kit, with a concept in mind, but no expertise ?

I own a renewable energy startup, and funding is so difficult. Hence due to the startup nature, lack of capital especially, I've only handled low profit projects and using personal funds. I have...

30 March 2020 8,565 2 View

How to calculate cell doubling time from confluecy of cells?

If i am Knowing the confluency of cell culture, is it possible to calculate the doubling time of cells.

23 December 2019 7,456 4 View

What is your hobby? How do you get enjoyment through it?

Almost all of us have one or more hobbies. These vary from person to person, e.g. singing, painting, writing, reading books, net surfing, gossiping, social media, etc. We enjoy ourselves by...

20 October 2019 2,374 11 View

If some paper have good reputation, SCI index and my paper has been accepted in this journal.now they are asking publication charge. should I publish?

If some paper have good reputation, SCI index and my paper has been accepted in this journal. Now they are asking publication charges. should I publish?

24 July 2019 8,591 5 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

2D or 3D phased array antennas??

Dear All, I will start my PhD maybe this year regarding phased array antennas. I'm interested in to find more detailed information about 3D phased array antennas and 2D phased array antennas. Then...

21 February 2021 368 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

Cytokine levels in tears sample?

Hi dear, Do you have any idea of a commercial lab who measure the cytokines levels in tears by multiplex or flow cytometry? Thank you

18 January 2021 5,928 3 View

Did I dilute my supernatant too much so cytokines will be below the limit of detection on a Luminex assay?

I am performing an experiment analyzing the cytokine production of macrophages in response to viral infection. I recently harvested and cultured peritoneal macrophages from mice. I plated these...

17 January 2021 5,516 3 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View