How do I prepare Standard Succinate Media?

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Cell optimization in 1D Nanotubes?

Dear All, I use Quantum Espresso. I want to optimize the structure of the carbon nanotube using the vc-relax. I got confused about how to fix cell_dofree here. For 3D, cell_dofree = 'all' or...

22 July 2024 5,708 0 View

I want to plot calibration curve of pure mirtazapine powder using HPLC. Can someone please suggest a method without HPLC buffer as a mobile phase?

I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...

14 July 2024 1,493 3 View

Error of Reshape the data in Pythonplot code?

I am trying to plot using the attached file data file, "wan_band.dat". In the past, I also found the same error. But somehow it was resolved automatically. Again, I stuck with it. I am not able to...

09 April 2024 4,738 1 View

What are the diffrent nonpharmacological treatments mentioned in Ayurveda being in practices ?

List of Nonpharmacological treatments modules cost-effectiveness practices PMC publications

06 February 2024 9,814 2 View

HEK293T cells electroporation?

If anybody has successfully done electroporation of RNA into HEK293T cell, kindly share the electroporation parameters. Or, if anyone is using Maxcyte STx machine for electroporation, please share...

28 January 2024 8,008 0 View

Is blood isolated monocyte can be kept in incubator in which cancer cell lines are growing?

Dear researchers kindly let me know is it will be feasible to culture blood cells in animal cell culture lab and put the isolated cells to grow in cell culture medium in the same CO2 incubator in...

06 January 2024 1,189 5 View

How to prepare fecal slurry/water?

In our ongoing study we aim to assess the fecal microbiome and metabolome in patients in Indian population. For achieving the same we are referring to Franzosa Eric et al's., study entitled "Gut...

11 December 2023 8,754 0 View

Why no RNA formed?

I have isolated plasmid and then linearised it with re. Then I put in-vitro transcription of the linearised plasmid but not band of mRNA could be observed only the linearised plasmid was seen....

19 November 2023 3,607 7 View

How shall I proceed with large plasmids?

What size of plasmid can be isolated from qiagen miniprep and midiprep kit, respectively? And at what temperature shall I grow a 36kb plasmid with 12.5ug/ml chloramphenicol resistance?

07 September 2023 2,600 3 View

Why no bands on Restriction Digestion of 36kb plasmid??

I am trying to isolate BAC plasmid of size 36kb. I tried with Qiagen Miniprep kit and Zymo BAC purification kit and ran on 0.5% gel. The bands can be seen on the gel but no bands were after I did...

31 August 2023 2,932 4 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

15 July 2024 9,025 3 View

What is the method of standard curve of IAA preparation?

Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with...

29 June 2024 4,421 1 View

How can I identify products of methyldopa oxidation by simple methods?

In alkaline solution (NaOH 0,1 M), methyldopa generates orangish solution. I suspect it turns into indolic compounds by base catalysed oxidation (possibly oligomers). How can I confirm...

01 April 2024 8,298 0 View

Does Azospirillum has a role on phosphorus mobilization and uptake in spring maize? If yes, which strains?

In context of undergraduate research on PUE and azospirillum inoculation in maize.

20 March 2024 5,084 0 View

Seeking recommendations for an FDA-approved antiepileptic drug containing an indole nucleus in its structure?

Hello RF Members, Seeking recommendations for an FDA-approved antiepileptic drug containing an indole nucleus in its structure. Thank you.

27 January 2024 1,182 0 View

Coliform, Fecal Coliform, E.coli – SMEWW 9221: Multiple-tube fermentation technique 3 or 5 sample-portions? How to combine E.coli results?

1.1. 3 or 5 serial sample-portion (dilutions) SMEWW 9221C indicates the use of 3 and 5 sample sample-portion (10, 1, 0.1 ml). As I understand, both are fine. If using 3, you can dilute the sample...

21 December 2023 7,151 0 View

Which medium is the best to be used for qualitative estimation of IAA production of PGPR?

As per the tiltle said, I want to qualitatively test how much IAA can be produced by some of the bacteria that I have just isolated from a soil sample. After culturing the soil bacteria in a...

03 December 2023 4,408 1 View

How to do N-Methylation of Indole?

I need to substitute N position with ethyl or methyl groups. How can I achieve this with good yield?

25 September 2023 7,446 1 View

Gram negative short curved rods, Indole negative, oxidase positive,luminescent strain with yellow morphology on TCBS?

Dear researchers, 1. What could be the isolate that is gram negative short curved rods, indole negative, oxidase positive, luminescent strain that grows on TCBS with yellow colony morphotype? Need...

22 September 2023 7,338 0 View

How can synthesis 4-bromo indole and 4-methyl indole?

Hi Respected RG member, I have a question. How can I synthesize 4-bromoindole and 4-methylindole? Thanks!

06 May 2023 7,410 5 View

Muhammad Tanweer Khan

https://www.researchgate.net/publication/6585421_Targeted_engineering_of_Azospirillum_brasilense_strain_SM_with_Indole_Acetamide_pathway_for_IAA_over-expression

Article Targeted engineering of Azospirillum brasilense strain SM wi...

Shrawan Kumar Upadhyay

Thanks for the Paper! It really helped me.