How to do N-Methylation of Indole?

Akilesh Manimuthu @Akilesh-Manimuthu

26 September 2023 1 7K Report

I need to substitute N position with ethyl or methyl groups. How can I achieve this with good yield?

Robert Adolf Brinzer

Using alkyl halides would probably be the easiest.

Badges
Science topic

Similar topics
Chemistry
Chemicals
Solvents

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

P53 detection in methylated and non methylated samples?

Hello everyone. I am about to search for the expression of genes including p53 in methylated and non methylated HPV samples. Should I extract DNA from the methylated samples and use SYBR green...

04 February 2021 2,749 3 View

Can someone explain why a methyl in phentolamine mesylate presents two correlations for 13C-HSQC, around the region it should appear?

Instead of appearing one single correlation of the signal of the carbon at 20.9 ppm (#11 in the attached figure), aligned to hydrogen at 2.29 ppm, it appeared two correlations (2,32 and 2,26 ppm)....

16 January 2021 4,774 2 View

Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

HPLC separation for methyl salicylate, camphor, thymol and menthol?

I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2...

28 December 2020 4,426 6 View

How to read an HPLC report?

I have four HPLC graphs (for methyl ethyl ketone (MEK) and sulfuric acid and little organic monomers) and I want to analyze them. Is there anyone who can help me with this?

23 December 2020 6,180 3 View

Is there any GTEx like web-based tool that may predict DNA methylation associated gene expression?

I am looking for an online tool to see whether a certain methylated CpG site can affect the expression of a certain gene or not?

18 December 2020 7,575 1 View

Can I use conventional PCR master mix instead of hot star Taq polymerase in DNA methylation studies or in bisulphite converted DNA ?

I am trying to predict age from DNA methylation. I am using conventional PCR master mix, but I didn't get my PCR product. Is it due to Master Mix? Should I use HotStar Taq Master Mix ?

08 December 2020 8,776 4 View

Do you know how to seperate GC peaks btw MMA (methyl methacrylate) and 1,4-dioxane ?

Want to know the method for the seperation of GC peak btw MMA and dioxane which are showing the same B.P. like 101oC

15 November 2020 691 3 View