Dear Darshana Deka thank you for sharing this very interesting chemical question with the RG community. The preparation of the cupric acetate - pyridine reagent (CAPR) is in fact quite easy and straightforward. A detailed protocol is given in the following relevant literature reference:
A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method
This paper is freely accessible as public full text (please see the attached pdf file). It is stated here that (ctation): "Cupric acetate pyridine reagent CAPR was prepared by placing different amounts of cupric acetate (2.5 g, 5 g, and 7.5 g) into a 100-mL volumetric flask and bringing to volume with water. Then, the content was swirled to dissolve the cupric acetate, and then it was filtered through Whatman no. 1 filter paper. Finally, the pH of the solution was adjusted to different values of 6.0, 7.0 and 8.0 using pyridine". This clearly shows that you can also use copper(II) acetate monohydrate for the CAPR preparation since the salt is dissolved in water anyway. Thus it doesn't matter if you use anhydrous or hydrated copper(II) acetate as starting material. You just need to adjust the stoichiometric amounts accordingly.
Good luck with your experiments and best wishes, Frank Edelmann