Darshana Deka

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Questions related from Darshana Deka

I am trying to perform SDS for my hydrolysed protein. But the sample is not getting seperated. Can anybody suggest what maybe the issue?

I have tried using 12% resolving gel as well as 10% resolving gel and 5% stacking gel combination. I have also run the marker which separated fine. But why can I not see bands for my sample? i...

01 November 2024 2,875 4 View

On aqueous extraction of oil what is the white milky layer that is obtained as supernatant?

I am trying to extract lipids using water as solvent. After extraction followed by centrifugation, I did not obtain any oil layer. Instead the supernatant was milky white, which I think might be...

31 October 2023 9,217 1 View

What is the reason for the base line shift in this GC-FID chromatogram?

This is the chromatogram that I've received after running Supelco C8-C24 FAME mix in a TG-5MS (30m*0.25µm*025µm) of 5% phenyl methyl polysiloxane column. Kindly suggest the reason behind the...

31 October 2022 3,002 5 View

How do i prepare cupric acetate- pyridine reagent (CAPR)?

I need CAPR reagent for free fatty acid analysis. Can copper(II) acetate monohydrate be used for its preparation? Thanks in advance.

07 February 2022 244 1 View

Antioxidant activity in linoleic acid system.

Has anyone performed assessment of Antioxidant activity in linoleic acid system? i followed this procedure Samples were dissolved in 0.1 M sodium phosphate buffer at pH 7.0, while BHT and BHA...

01 January 1970 9,053 1 View