Hello. I need to 100 ul from 75mg/ml polyethyleneimine solution (M.N. 60000 and 50 wt.% aqueous solution). Its a very viscose liquid and with CAS No [9002-98-6].
I have 3 challenges:
1-what is M.N 60000?
2-How much is PEI density?
3-How can I aspirate PEI solution with a micropipette? Do I have to heat PEI?
Thank you for taking time.
Hi Zahra.
1)
What is MN and Mw in GPC?
Mn is the number averaged MW, and Mw is the weight averaged MW. The midpoint of the distribution in terms of the number of molecules is Mw. The third moment, Mz, has more weighting with regards to higher MWs. The Mw:Mn ratio is termed as polydispersity, and is used for describing the distribution width.29 de jul. de 2015
GPC stands for Gel Permeation Chromatography, a technique to obtain the molecular weights of polymers.
For more details, check: https://www.azom.com>article
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2)
POLYETHYLENEIMINE Properties
Melting point
59-60°C
Boiling point
250 °C(lit.)
Density
1.030 g/mL at 25 °C
vapor pressure
9 mmHg ( 20 °C)
refractive index
n 20/D 1.5290
For more details check: https://www.chemicalbook.com › ... › CAS DataBase List
3)
I guess you could heat up the PEI solution mildly for sampling with a pipette.
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