I have a bunch of Z-stacks that have a red, green, and blue. I want to merge the red and green signals only and have a maximum intensity projection of the combined output of red and green only, not blue, and shown as only 1 signal?.
Dear Neal Edward Beeman,
I would like to clarify what you are looking for:
1) You have R,G,B channels with their own value and you want to keep only the R and G ones ?
=> If it is that, in Fiji you can go to Image/Color/Split channels, and you will have the three channels separated, you can then go to Image/Color/Merge channels, select only your R and G channels. (if you have many stacks, you could automatically perform that, let me know I can give you a script for that)
Small note: if you do not have the separated channels in your stack, you can first convert it as a composite from Image/Color/Make composite.
2) Then you want to perform a maximum projection through your stack for each channel separately ? Or you would like the maximum projection through the stack for the maximum of either R or G channel ?
=> For the first one, you can just go to Image/Stacks/Z project, select sum projection and you are all set.
Let me know if it solves your issue or if I can be of further help.
Best,
Christopher
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