How do I measure LWP in sorghum? Is it proper to use the pressure bomb technique?

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David W. Lawlor

Hello,

measurements of leaf water potential can be easily made on monocots such as sorghum. Very quickly cover the leaf (it can be the whole leaf or part) with `saran wrap' or similar film, immediately cut the leaf with a sharp blade, place in the seal with the cut end just exposed so that it can be seen clearly with a hand lens, tighten the seal, and apply pressure slowly (tighten seal as required to stop leaks). Watch until sap just exudes from the end: record the pressure. Follow the safety instructions with the pressure chamber very carefully taking particular care to protect yourself: pressure chambers are potentially dangerous.

Regards

Jose Kallarackal

Since Sorghum leaves are flat, you will require a special seal (which is available with most manufacturers) for getting a system without leakage. The usual seal is for cylindrical petioles, which is not suitable for your case. In case the above type of seal is not available, a psychrometer type water potential measurement system is useful, however, the pressure chamber is very fast and easy to use.