It is practically impossible to prepare such solution from the mentioned stocks. Although there are three alternative methods:
1. Use pure materials (not stock solutions)
2. Mix 1 ml of each stock solution (esterone and triclosan) and add 8 ml 1.5 ug/ml ACN solution to have a final concentration of both esterone and triclosan and 1.2 ug/ml ACN.
3. If possible, you can also add 8 ml of a higher concentration (about 1.85 ug/ml) of ACN solution to 1 ml of each stock solution.
100 mg/l = 100000ug/l= 100 ug/ml which is 10 times of 10ug/ml.
so take 1 ml from the stock solution of 100 mg/l into 10 ml of volumetric flock and make up the volume. concentration of final solution will be 10ug/ml. for 1.5 ug/ml, take 1.5 ml from stock solution of 10ug/ml in 10 ml volumetric flask and make up the volume. using both stock solution you can prepare various dilutions
can we use simple V1S1=V2S2 formula here ? here V1 =vol. of stock sol we need to add S1= conc. of stock i.e,100mg/l or 100ug/ml V2=vol. of final sol. which is 1ml S2=conc. of final sol. that is 10ug/ml. if we calculate this by using v1s1=v2s2 we will get 100ul . this 100 ul should be added to the final solution from stock
Kovan Ismael well it depends how much final volume you need, lets say you are going to make solution with a 30ml final volume with a concentration of 100ug/ml from a concentration of 20mg/ml (Proteinase K most probably).
so.! first adjust all the units to be the same like for 100ul convert it to mg which is 0.1mg/ml.... Now.!
C1V1C2V2 where C1=20mg/ml, V1=?,, C2=0.1mg/ml and V2=30ml.
20x?=0.1x30,
3/20=0.15 or 150ul.
So, take 150 uL of your 20 mg/mL stock, and add it to final volume ( 30 mL for practical purposes) of your diluent.