How do I know if my plasmid is a high or low copy number type?

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Rahmat Azhari Kemal Popular answer

You can check the plasmid's origin of replication.

http://blog.addgene.org/plasmid-101-origin-of-replication

Rahmat Azhari Kemal

Demo Yamane Tesfaye

https://www.qiagen.com/no/resources/faq?id=1f42840e-fbd7-4734-b0cd-e17372a9e5a4&lang=en

Aswathy Narayanan

Check the ori of the plasmid. And,Ii there are centromeric sequences as in some off the yeast vectors, they are low copy.

R. M. Mehdi

The copy number of a plasmid is depending on the origin of replication (ori) they contain.
The different oris regulate the replication mechanism of the plasmid. High-copy plasmids often contain mutated versions of an ori.