I want to design construct on my selected vector.However how do I know whether my plasmid is high or low copy number?
You can check the plasmid's origin of replication.
http://blog.addgene.org/plasmid-101-origin-of-replication
https://www.qiagen.com/no/resources/faq?id=1f42840e-fbd7-4734-b0cd-e17372a9e5a4&lang=en
Check the ori of the plasmid. And,Ii there are centromeric sequences as in some off the yeast vectors, they are low copy.
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