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Questions related from Anteneh Hailu Abebe
When I grow E,coli stain on solid support( i.e Agarose) for time lapse microscopy Experiment , I found fast in growth relative to batch culture in broth media. I tried to maintain all condition...
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I want to design construct on my selected vector.However how do I know whether my plasmid is high or low copy number?
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To enhance the efficiency of FITC staining, I would like to use toluene as permeability agent. But I can't get the working protocols for E. coli cells. Is there anyone who can explain to me how to...
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I expressed my protein with GFP in the bacterial chromosomes. To measure the intensity of expressed protein signal and to determine the threshold level, I need to stain the bacteria by FITC. So...
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I have run the PCR for several times getting the same light band results. I have also tried using more of DNA template but still the concentration is too little. Is there any mean to get more PCR...
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