Anteneh Hailu Abebe

5 Questions 7 Answers 0 Followers

Questions related from Anteneh Hailu Abebe

Why some strain of E.coli grow fast on solid support than batch culture?

When I grow E,coli stain on solid support( i.e Agarose) for time lapse microscopy Experiment , I found fast in growth relative to batch culture in broth media. I tried to maintain all condition...

08 August 2016 4,012 10 View

How do I know if my plasmid is a high or low copy number type?

I want to design construct on my selected vector.However how do I know whether my plasmid is high or low copy number?

13 March 2016 4,248 5 View

How can I use toluene to treat E.coli cells for FITC Staining?

To enhance the efficiency of FITC staining, I would like to use toluene as permeability agent. But I can't get the working protocols for E. coli cells. Is there anyone who can explain to me how to...

05 January 2016 5,316 1 View

Does anyone know how to stain bacteria with Fluo-rescein isothiocyanate (FITC)?

I expressed my protein with GFP in the bacterial chromosomes. To measure the intensity of expressed protein signal and to determine the threshold level, I need to stain the bacteria by FITC. So...

27 December 2015 1,539 4 View

What DNA concentration do I need for DNA sequencing?

I have run the PCR for several times getting the same light band results. I have also tried using more of DNA template but still the concentration is too little. Is there any mean to get more PCR...

16 December 2015 2,783 6 View