I want to extract the bacterial DNA from saliva by CTAB method, Do any body have the solution?
Here is the reference to CTAB method used for isolating plant and bacterial DNA.
http://nar.oxfordjournals.org/content/8/19/4321.abstract
I assume the it is identical or very similar solution used for saliva. Keep in mind that the amount of bacteria present in saliva will be very less so you will have only limited amount of bacterial DNA isolated. I assume it will only be sufficient for PCR based analyses and nothing more.
i was using the DNA extraction protocol from blood but it seems that it is not working.. one problem is that the in what form the sample should be, either the washouts with saline solution or spitting sample will be ok.
it may be that the amounts of dna are too small to precipitate properly so you could try adding a co-precipitant like glycogen or rna just prior to final precipitation. This gives the bulk to spin down and carries the dna of interest with it and everything will stick well to the bottom of the tube. Glycogen is probably better as it has no impurities to interfere with pcr in the later stages
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