My aim is to look at cytokine response (IL-1b, TNF-a, IL-6) in THP-1 cell supernatant after infection with GBS. Briefly, I inactivated GBS using formalin and incubated differentiated THP-1 cells at MOI 10:1 (1, 3, 24h), collected cell supernatant and used an ELISA kit to determine cytokine expression - no difference between control and infected.