How do I identify Ralstonia solanacearum using a DNA marker and real time PCR?

More Aziz Ahmed Ujjan's questions See All

In your experience what is the difference between G.652D and G.655D optical fiber. In field and in lab?

In our current era there is a big confusion about the usage of G.652 and G.655 optical fiber cable. Theoretically G.655 is much better than G.652 but the operator feel that G.652 is giving almost...

11 February 2021 4,006 8 View

Why is there no global interest in using alternatives to rebar?

Why is there no global interest in using alternatives to rebar? GFRP has many advantages such as 1-High strength. 2-Corrosion resistance 2-High Insulation. it is better than Steel Rebars?

24 January 2021 5,757 6 View

How to plot a hardness mapping reading using origin software?

Hi, I have a rectangular sample and a set of hardness distribution readings throughout the sample. Can anyone here help me, how to map it using Origin software? Thank you.

02 January 2021 4,079 3 View

Are the same rapid and clinical test of diabetics?

28 December 2020 9,109 12 View

Why Riemann Zeta Function is important ?

How was the importance of the zeta function discovered ? why do zeta function contain so much information ? What other areas of mathematics does it relate to ? Are there any books on the RH...

28 December 2020 3,128 5 View

What are the causes of low pressure in human body?

28 December 2020 8,183 23 View

What are the effect of low pressure in human body?

28 December 2020 876 6 View

What we learn from Zee Bangla TV or Star Jolsa TV channel?

27 December 2020 9,357 3 View

What counts as publication in scholarly journals?

Hello Seniors! Please guide me, what counts as a publication in a scholarly journal? e.g. book reviews etc.

24 December 2020 10,038 1 View

Does book Review in scholarly journals counts as a publication?

Does book Reviews written for scholarly journals or in a magazine count as a publication?

24 December 2020 2,265 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Adam Okorski

I think, You can use qPCR primers and probe described in the publication (http://www.jmb.or.kr/journal/download.php?Filedir=../submission/Journal/020/&num=3762). Best reagards.

Adam

Alex Ignatov

Aziz, RT-PCR alone will not be reliable identification for Rs. You should use all steps described by EPPO or APHIS protocols, like http://archives.eppo.int/EPPOStandards/PM7_DIAGNOS/pm7-21(1).pdf

http://caps.ceris.purdue.edu/webfm_send/1610

http://www.ars.usda.gov/SP2UserFiles/Place/00000000/opmp/RalstoniaR3b2May2010.pdf

Aziz Ahmed Ujjan

Alex Ingatov, thanks for good suggestion, if i can purchase the specific primers