30 November 2017 0 5K Report

Some of my packaging cell line (GP2-293) have peeled of into the media containing the lentivirus which they have produced. If I spin down the tube, will the lentivirus also go to the pellet?

I want to have media containing just the lentivirus to transduce my target cells. If there are packaging cells present in the media, I run the risk of contaminating my cell line.

I am hoping that only the cells will go to the pellet and the virus will stay suspended. What speed should I spin down at? Are there other filtration methods to get the cells out?

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