How do I figure out how much template I need to add for my PCR reaction?

03 March 2015 0 3K Report

I need to have a final concentration of 500ng of cDNA in a 25ul reaction. Right now I have 950ng/ul. what is the math involved? What is a good cDNA final concentration to have for qRT-PCR? The kit I have asks for no more than 500ng.

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