How do I efficiently use the Bioanalyzer?

More Boniface Kimathi's questions See All

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Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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Runs of nucleotides in Sanger sequencing?

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How to improve RNA isolation?

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I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

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What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

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Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

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Michail Yekelchyk

Hi,

Unfortunately, Agilent BioAnalyzer chips are strictly single-use. Alternatively, you can store the samples until you have enough to run the whole chip. From other hand, some machines like PerkinElmer LabChip allow to use the chips for up to 500 samples by simply adding the gel/marker solutions. Really high throughput machines like the Fragment Analyser allow to prepare the solutions in tubes/flasks and use them for hundreds of samples.

Best,

Michail

Boniface Kimathi

Thank you for your answers Mathew A Beale and Michail Yekelchyk . Once a lab has acquired an equipment, it is not easy to change to a new technology. The above suggestions are nice but it would have been nice if I could efficiently use the Bioanalyzer that we already have in the lab. Everything about it is alright, its just the chips with less than full samples that I was worried about.

Anna Nieborak

Hi Boniface,

Have a look at this article: Article Re-Use of Commercial Microfluidics Chips for DNA, RNA, and P...

A former postdoc from our lab used to re-use Bioanalyzer chips (at least the ones for DNA) and it was working fine. It seems like with the RNA chips it's a bit more of a hassle but I think it's worth trying.

Hope it is of any help.

Anna

Hi Anna Nieborak

Thanks for the suggested read. I will go through it and see if the reused chips are a good way to go. We have had to use less than a full chip when we have few samples to run.