Hi, I want teratologists/embryologists to help me explain these features I encountered in mice Fetus below?

More Boniface Echefu's questions See All

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How do I efficiently use the Bioanalyzer?

Hi, I am preparing libraries for transcriptome sequencing. The kit recommends using the Bioanalyzer at various steps. Is there a way I can re use the chips? I find it a waste when am doing only...

20 September 2018 536 4 View

Anybody culturing human ovarian epithelial cell?

I need immortalized ovarian surface epithelial cells for culture. Any help will do. Thanks

19 August 2018 4,442 0 View

How is a t-test on fold change calculated?

How does one determine whether a fold change calculated on qPCR data using 2-ΔΔCt method is significant? Answers provided to a similar question earlier indicate that one should do a one sided...

28 October 2014 3,170 2 View

How do I avoid formation of polymers of my protein of interest in Western Blot?

I have done western blots to detect a 19kDa protein. I am getting signals representing polymerization of the protein.This would not have been a problem but there is very little or no signal where...

25 March 2014 2,256 4 View

Can someone suggest how to get a consensus sequence for a gene?

I am trying to get the consensus sequence from the same genes so that i can use it to design primers. I have aligned them in ClustalO and can see the sequence in JalView. How do I export it to a...

29 July 2013 9,871 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Gbenga Peter Oderinde

Have you tried checking through the groups because this might possibly be the sac

Boniface Echefu

It's not sac, I think. These look like embryos.

Hector Oviedo-Cruz

It is the Nuchal Translucency [MeSH], and its thickness is associated to the presence of congenital abnormalities.Article Fetal nuchal edema and developmental anomalies caused by gen...

Thank you for this contribution, Hector Oviedo-Cruz . I also appreciate the inclusion of an article.