Boniface Kimathi

4 Questions 7 Answers 0 Followers

Questions related from Boniface Kimathi

How do I efficiently use the Bioanalyzer?

Hi, I am preparing libraries for transcriptome sequencing. The kit recommends using the Bioanalyzer at various steps. Is there a way I can re use the chips? I find it a waste when am doing only...

21 September 2018 507 4 View

How is a t-test on fold change calculated?

How does one determine whether a fold change calculated on qPCR data using 2-ΔΔCt method is significant? Answers provided to a similar question earlier indicate that one should do a one sided...

29 October 2014 3,145 2 View

How do I avoid formation of polymers of my protein of interest in Western Blot?

I have done western blots to detect a 19kDa protein. I am getting signals representing polymerization of the protein.This would not have been a problem but there is very little or no signal where...

26 March 2014 2,228 4 View

Can someone suggest how to get a consensus sequence for a gene?

I am trying to get the consensus sequence from the same genes so that i can use it to design primers. I have aligned them in ClustalO and can see the sequence in JalView. How do I export it to a...

30 July 2013 9,842 2 View