This is a picture about IHC result. We found that the protein was highly expressed in the nuclear and cytoplasm of spermatocyte, round spermatids, spematogonia and Sertoli cells, but was hardly detectable in elongated spermatozoon. I am confuesed how to distinguish second spermatocytes and round spermatids.
I agree with Laura and Shahzad, it is not easy to detect secondary spermatocytes, specially when tubular sections are not completely obliques (since maturation stages are mixed). Performing complementary histological sections with PAS staining could help you to differentiate stage XII, and thus secondary spermatocytes. But tubules must be completely rounded and with the lumen visible. You can look up my last work in which I combine immunohistological techniches with PAS staining in mouse testis.
Bin, the best way is to just focus on round spermatids, as they can easily be identified because of their peculiar small size and location. And i totally agree with Laura's comment, Secondary spermatocytes are rarely seen and even if seen needs a very expert eye for detection. You can also easily identify primary spermatocytes because of their size and location.
I agree with Laura and Shahzad, it is not easy to detect secondary spermatocytes, specially when tubular sections are not completely obliques (since maturation stages are mixed). Performing complementary histological sections with PAS staining could help you to differentiate stage XII, and thus secondary spermatocytes. But tubules must be completely rounded and with the lumen visible. You can look up my last work in which I combine immunohistological techniches with PAS staining in mouse testis.
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