I work on plant extract for seed germination assay. However, I face some issues on dissolving my plant extract. Initially, I try to use distilled water to dissolve it but my plant extract is not dissolved completely. So, I am planning to use DMSO and dilute DMSO at 1% using distilled water to dissolve my sample extract. May I know is this concentration toxic to seed germination? Or dissolve sample using pure DMSO then only do serial dilution? Any suggestion? Thanks in advance!
Lim Boon Chin
Dear Dr Ikbal Chaieb, thank you so much for your answer. I will try them out. Thanks.
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Abolfazl Shirmohamadi
you can use the DMSO in higher concentration up to even 10% but it depend on your test. be careful about control test.
if your problem did not solve you can use DMF as mentioned above.
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