Ca2+ binding protein with suspected GTPase activity. Experiment Shows hydrolysis of GTP and also peak of inorganic phosphate on NMR.
You could try a specific inhibitor for you GTPase in your assay. If these are cells, you could add the drug to the culture medium and after the incubation test various parameters.
Cdc42 inhibitor (ML-141), Rac inhibitor (NSC or EHT1864) and RhoA inhibitor (Rhosin) are all available commercially.
Thank you Elena for your inputs.
I forgot to mention that it is a protein localized within the nucleus and hence the drug titration isn't an option. Furthermore, I had tried it with a mutant variant of the same, with calcium binding site disabled, but NMR didn't give me good results.
You could mutate some conserved residues e.g. the Ser or Lys in the P-Loop.
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